....The confocal microscopy core facility at the Rheumatology, Immunology and Allergy division of Brigham and Women's Hospital performs widefield & confocal microscopy, photobleaching, and live cell imaging studies.

New users: visit here to learn useful information such as our instruments, policies, and how to make an appointment.

The guides section will provide protocols, catalogue numbers, and suggestions to help you achieve high-quality imaging while avoiding common pitfalls.


Manager:
Tyler Hickman

Email:  thickman@rics.bwh.harvard.edu

Director: Nancy Kedersha, Ph.D.    

 


Announcements:

1-8 July
Tyler will be on vacation. Tech-assisted work is unavailable. Users who are not approved for off-hours use (Senior users) should let Nancy know if you plan to use the scope during this time.

1 June 2009
We have a new 488 laser. It is quite powerful and photobleaching experiments are now much easier.

28 April 2009
We are currently looking for a new manager/technician for the confocal core to begin ASAP. Please contact Nancy and Tyler for details.

1 April 2009
We have installed a new 60x objective while our original 60x is being repaired. Please use extra care when using this objective!

1 December 2008
There will be limited availability for independent and tech-assisted users from December 17-26. Nancy Kedersha should be contacted with specific questions and troubleshooting during this time.

24 November 2008
New rates will be in effect beginning December 1.

A MetaMorph image analysis workstation has been added for data acquired at this core. Please email for access.

3 July 2008
Confocal startup, shutdown, and ImageJ file import guides have been added to the "Guides" section. A ImageJ file import plugin has been added to the links section.

23 June 2008
The shedule guide has been updated to answer some commonly-asked questions. The links have been updated with catalogues for useful items such as fluorescent secondary antibodies and precision tweezers.

7 February 2008
Our confocal system is being upgraded with new lasers and additional laser controls.  This will allow precise laser power for use in FRAP and FLIP experiments. The system will become available again Thursday February 14th.

10 January 2008
A new custom-designed microscope incubator has been installed on our microscope. CO2 control will be added soon. Independent users: please see me before arriving at a new appointment to learn how to operate the microscope without affecting the enclosure.

19 December 2007
A new microscope incubator is scheduled to be installed the week of January 7. Updates will be posted here.

4 December 2007
A network server named "confocal" is now available for all RIA users to transfer their files within the RIA network.